Transcription factor expression in lipopolysaccharide-activated peripheral-blood-derived mononuclear cells.

ABSTRACT: Transcription factors play a key role in integrating and modulating biological information. In this study, we comprehensively measured the changing abundances of mRNAs over a time course of activation of human peripheral-blood-derived mononuclear cells ("macrophages") with lipopolysaccharide. Global and dynamic analysis of transcription factors in response to a physiological stimulus has yet to be achieved in a human system, and our efforts significantly advanced this goal. We used multiple global high-throughput technologies for measuring mRNA levels, including massively parallel signature sequencing and GeneChip microarrays. We identified 92 of 1,288 known human transcription factors as having significantly measurable changes during our 24-h time course. At least 42 of these changes were

SUBMITTER: Roach JC 

PROVIDER: S-EPMC2042192 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature