Stimulus-dependent differential regulation in the Escherichia coli PhoQ PhoP system.

ABSTRACT: In Escherichia coli, Salmonella, and related bacteria, the PhoQ-PhoP system regulates the expression of a large collection of genes in response to conditions of low magnesium or to the presence of certain antimicrobial peptides. We measured transcription of four PhoP-regulated promoters in E. coli that have significantly different PhoP-binding sites. Surprisingly, three promoters show identical responses to magnesium concentrations that range over four orders of magnitude. By analyzing and testing a simple model of transcriptional regulation, we find an explanation for this puzzle and show that these promoters are indeed differentially regulated at sufficiently high levels of stimulus. We then use this analysis to infer an effective level of phosphorylated PhoP as a function of magnesium s

SUBMITTER: Miyashiro T 

PROVIDER: S-EPMC2042202 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature