Thy-1 is expressed in hepatic myofibroblasts and not oval cells in stem cell-mediated liver regeneration.

ABSTRACT: Thy-1, a marker of hematopoietic stem cells, has been reported to be expressed by oval cells proliferating during stem cell-mediated regeneration in rat liver, suggesting a relationship between the two cell populations. Consequently, Thy-1 has become an accepted cell surface marker to sort hepatic oval cells. In the present study we used the well-characterized 2-acetylaminfluorene/partial hepatectomy model to induce transit-amplification of hepatic oval cells in the regenerating liver and characterized Thy-1 expression using Northern hybridization, quantitative reverse transcriptase-polymerase chain reaction analysis, immunofluorescence confocal microscopy, and immunoelectronmicroscopy. We found that Thy-1 expression was induced during transit-amplification of the oval cell population, but

SUBMITTER: Dezso K 

PROVIDER: S-EPMC2043514 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature