DNA pyrosequencing-based bacterial pathogen identification in a pediatric hospital setting.

ABSTRACT: Sole reliance on biochemical methods can limit the clinical microbiology laboratory's ability to identify bacterial pathogens. This study describes the incorporation of DNA pyrosequencing-based identification for routine pathogen identification of atypical clinical isolates in a large children's hospital. The assay capitalized on the highly conserved nature of 16S rRNA genes by positioning amplification and sequencing primers in conserved target sequences flanking the variable V1 and V3 regions. A total of 414 isolates of 312 pediatric patients were tested by DNA pyrosequencing during the time period from December 2003 to July 2006. Seventy-eight different genera were specified by DNA pyrosequencing, and isolates were derived from diverse specimen types. By integrating DNA sequencing of ba

SUBMITTER: Luna RA 

PROVIDER: S-EPMC2045277 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature