Comparison of the contributions of the nuclear and cytoplasmic compartments to global gene expression in human cells.

ABSTRACT:

Background

In the most general sense, studies involving global analysis of gene expression aim to provide a comprehensive catalog of the components involved in the production of recognizable cellular phenotypes. These studies are often limited by the available technologies. One technology, based on microarrays, categorizes gene expression in terms of the abundance of RNA transcripts, and typically employs RNA prepared from whole cells, where cytoplasmic RNA predominates.

Results

Using microarrays comprising oligonucleotide probes that represent either protein-coding transcripts or microRNAs (miRNA), we have studied global transcript accumulation patterns for the HepG2 (human hepatoma) cell line. Through subdividing the total pool of RNA transcripts into samples from nuclei,

SUBMITTER: Barthelson RA 

PROVIDER: S-EPMC2048942 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature