Big results from small samples: evaluation of amplification protocols for gene expression profiling.

ABSTRACT: Microarrays have revolutionized many areas of biology due to our technical ability to quantify tens of thousands of transcripts within a single experiment. However, there are still many areas that cannot benefit from this technology due to the amount of biological material needed for microarray analysis. In response to this demand, chemistries have been developed that boast the capability of generating targets from nanogram amounts of total RnA, reflecting minimal amounts of biological material, on the order of several hundred or thousand cells. Herein, we describe the evaluation of four chemistries for RnA amplification in terms of reproducibility, sensitivity, accuracy, and comparability to results from a single round of T7 amplification. No evidence for false-positive measurements of di

SUBMITTER: Viale A 

PROVIDER: S-EPMC2062549 | biostudies-literature | 2007 Jul

REPOSITORIES: biostudies-literature