Single-cell-based sensors and synchrotron FTIR spectroscopy: a hybrid system towards bacterial detection.

ABSTRACT: Microarrays of single macrophage cell-based sensors were developed and demonstrated for potential real-time bacterium detection by synchrotron FTIR microscopy. The cells were patterned on gold electrodes of silicon oxide substrates by a surface engineering technique, in which the gold electrodes were immobilized with fibronectin to mediate cell adhesion and the silicon oxide background was passivated with polyethylene glycol (PEG) to resist protein adsorption and cell adhesion. Cell morphology and IR spectra of single, double, and triple cells on gold electrodes exposed to lipopolysaccharide (LPS) of different concentrations were compared to reveal the detection capability of this cell-based sensing platform. The single-cell-based system was found to generate the most significant and consi

SUBMITTER: Veiseh M 

PROVIDER: S-EPMC2065758 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature