Tagging of MADS domain proteins for chromatin immunoprecipitation.

ABSTRACT:

Background

Most transcription factors fulfill their role in complexes and regulate their target genes upon binding to DNA motifs located in upstream regions or introns. To date, knowledge about transcription factor target genes and their corresponding transcription factor binding sites are still very limited. Two related methods that allow in vivo identification of transcription factor binding sites are chromatin immunoprecipitation (ChIP) and chromatin affinity purification (ChAP). For ChAP, the protein of interest is tagged with a peptide or protein, which can be used for affinity purification of the protein-DNA complex and hence, the identification of the target gene.

Results

Here, we present the results of experiments aiming at the development of a generic tagging approa

SUBMITTER: de Folter S 

PROVIDER: S-EPMC2071916 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature