An evaluation of Comparative Genome Sequencing (CGS) by comparing two previously-sequenced bacterial genomes.

ABSTRACT:

Background

With the development of new technology, it has recently become practical to resequence the genome of a bacterium after experimental manipulation. It is critical though to know the accuracy of the technique used, and to establish confidence that all of the mutations were detected.

Results

In order to evaluate the accuracy of genome resequencing using the microarray-based Comparative Genome Sequencing service provided by Nimblegen Systems Inc., we resequenced the E. coli strain W3110 Kohara using MG1655 as a reference, both of which have been completely sequenced using traditional sequencing methods. CGS detected 7 of 8 small sequence differences, one large deletion, and 9 of 12 IS element insertions present in W3110, but did not detect a large chromosomal inversion

SUBMITTER: Herring CD 

PROVIDER: S-EPMC2072959 | biostudies-literature | 2007 Aug

REPOSITORIES: biostudies-literature