GATA-1 transcription is controlled by distinct regulatory mechanisms during primitive and definitive erythropoiesis.

ABSTRACT: Transcription factor GATA-1 is required for the terminal differentiation of both the primitive and definitive erythroid cell lineages, and yet the regulatory mechanisms of GATA-1 itself are not well understood. To clarify how the GATA-1 gene is transcriptionally controlled in vivo, presumptive regulatory regions of the gene were tested by fusion to a reporter gene and then examined in transgenic mice. We found that a transcriptional control element located between -3.9 and -2.6 kb 5' to the erythroid first exon serves as an activating element and that this sequence alone is sufficient to recapitulate the expression of GATA-1 (but uniquely in primitive erythroid cells). Addition of sequences from the GATA-1 first intron to this upstream element provides a necessary and sufficient condition

SUBMITTER: Onodera K 

PROVIDER: S-EPMC20749 | biostudies-literature | 1997 Apr

REPOSITORIES: biostudies-literature