New genera of RNA viruses in subtropical seawater, inferred from polymerase gene sequences.

ABSTRACT: Viruses are an integral component of the marine food web, contributing to the disease and mortality of essentially every type of marine life, yet the diversity of viruses in the sea, especially those with RNA genomes, remains very poorly characterized. Isolates of RNA-containing viruses that infect marine plankton are still rare, and the only cultivation-independent surveys of RNA viral diversity reported so far were conducted for temperate coastal waters of British Columbia. Here, we report on our improvements to a previously used protocol to investigate the diversity of marine picorna-like viruses and our results from applying this protocol in subtropical waters. The original protocol was simplified by using direct filtration, rather than tangential flow filtration, to harvest viruses fr

SUBMITTER: Culley AI 

PROVIDER: S-EPMC2074930 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature