Novel nickel resistance genes from the rhizosphere metagenome of plants adapted to acid mine drainage.

ABSTRACT: Metal resistance determinants have traditionally been found in cultivated bacteria. To search for genes involved in nickel resistance, we analyzed the bacterial community of the rhizosphere of Erica andevalensis, an endemic heather which grows at the banks of the Tinto River, a naturally metal-enriched and extremely acidic environment in southwestern Spain. 16S rRNA gene sequence analysis of rhizosphere DNA revealed the presence of members of five phylogenetic groups of Bacteria and the two main groups of Archaea mostly associated with sites impacted by acid mine drainage (AMD). The diversity observed and the presence of heavy metals in the rhizosphere led us to construct and screen five different metagenomic libraries hosted in Escherichia coli for searching novel nickel resistance determ

SUBMITTER: Mirete S 

PROVIDER: S-EPMC2075024 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature