Regulatory evolution in proteins by turnover and lineage-specific changes of cyclin-dependent kinase consensus sites.

ABSTRACT: Evolutionary change in gene regulation is a key mechanism underlying the genetic component of organismal diversity. Here, we study evolution of regulation at the posttranslational level by examining the evolution of cyclin-dependent kinase (CDK) consensus phosphorylation sites in the protein subunits of the pre-replicative complex (RC). The pre-RC, an assembly of proteins formed during an early stage of DNA replication, is believed to be regulated by CDKs throughout the animals and fungi. Interestingly, although orthologous pre-RC components often contain clusters of CDK consensus sites, the positions and numbers of sites do not seem conserved. By analyzing protein sequences from both distantly and closely related species, we confirm that consensus sites can turn over rapidly even when the

SUBMITTER: Moses AM 

PROVIDER: S-EPMC2077061 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature