Identification of differentially expressed genes in mouse Pax6 heterozygous lenses.

ABSTRACT:

Purpose

Pax6 is a critical regulator of the developing lens, other ocular tissues, central nervous system, and pancreas. Downstream targets of Pax6 are largely unknown. The present study was designed to identify differentially expressed genes in Pax6 heterozygous and normal mouse lenses.

Methods

RNAs from 8-week-old normal and Pax6 heterozygous mouse lenses were analyzed by both RT-PCR differential display and a candidate-gene approach. The expression levels of identified genes were confirmed by semiquantitative RT-PCR.

Results

Eight transcripts encoding phosphatase inhibitor Pip-1 protein, heat shock protein Hsp40, Purkinje cell protein Pcp4, an expressed sequence tag (EST; AA331381) originally reported in a brain-specific library, Pitx3, and CBP, were confirmed to b

SUBMITTER: Chauhan BK 

PROVIDER: S-EPMC2080866 | biostudies-literature | 2002 Jun

REPOSITORIES: biostudies-literature