Spectrum and range of oxidative stress responses of human lens epithelial cells to H2O2 insult.

ABSTRACT: PURPOSE:Oxidative stress (OS) is believed to be a major contributor to age-related cataract and other age-related diseases. METHODS:cDNA microarrays were used to identify the spectrum and range of genes with transcript levels that are altered in response to acute H(2)O(2)-induced OS in human lens epithelial (HLE) cells. HLE cells were treated with 50 microM H(2)O(2) for 1 hour in the absence of serum, followed by a return to complete medium. RNAs were prepared from treated and untreated cells at 0, 1, 2, and 8 hours after H(2)O(2) treatment. RESULTS:The data showed 1171 genes that were significantly up- and downregulated in response to H(2)O(2) treatment. Several functional subcategories of genes were identified, including those encoding DNA repair proteins, antioxidant defense enzymes, mo

SUBMITTER: Goswami S 

PROVIDER: S-EPMC2080867 | biostudies-literature | 2003 May

REPOSITORIES: biostudies-literature