Colocalization of transcription and translation within cytoplasmic poxvirus factories coordinates viral expression and subjugates host functions.

ABSTRACT: Poxviruses are large DNA viruses that include the causal agent of human smallpox and vaccinia virus. Poxviruses replicate in cytoplasmic foci known as DNA factories. Here we show that a virus-encoded transcription factor, viral mRNA, cellular RNA-binding protein heterodimer G3BP/Caprin-1 (p137), translation initiation factors eIF4E and eIF4G, and ribosomal proteins are concentrated in the same subdomains of cytoplasmic DNA factories. Furthermore, a cell coinfected with two recombinant vaccinia viruses expressing a virus core protein fused to cyan or yellow fluorescent protein displayed separate cyan and yellow factories, indicating that each factory formed from a single genome and was the site of transcription and translation as well as DNA replication. Hijacking of the host translation ap

SUBMITTER: Katsafanas GC 

PROVIDER: S-EPMC2084088 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature