Interaction of an amphipathic peptide with phosphatidycholine/phosphatidylethanolamine mixed membranes.

ABSTRACT: The effect of 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine (DOPE) in mixed membranes with 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) on interaction with a class A amphipathic peptide, Ac-DWLKAFYDKVAEKLKEAF-NH(2) (Ac-18A-NH(2)), was investigated. The fluorescence lifetime of 2-(9-anthroyloxy)stearic acid and (2)H NMR spectra were used to evaluate the penetration of water molecules into the membrane interface and the order of lipid acyl chains, respectively. The results demonstrated that DOPE in the mixed membranes decreased the fluorescence lifetime and increased the acyl-chain order, and that Ac-18A-NH(2) affected them more for membranes with higher DOPE fractions. The partition coefficient (K(p)) of the peptide to the mixed membranes was increased with the increase in the DO

SUBMITTER: Shintou K 

PROVIDER: S-EPMC2084244 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature