Mitochondrial positioning in fission yeast is driven by association with dynamic microtubules and mitotic spindle poles.

ABSTRACT: Microtubules mediate mitochondrial distribution in the yeast Schizosaccharomyces pombe and many higher eukaryotic cells. In higher eukaryotes, kinesin motor proteins have been shown to transport mitochondria along microtubules, but the nature of the mitochondria-microtubule interactions in S. pombe has not been explored. By time lapse, total internal reflection fluorescence microscopy, or spinning-disk confocal microscopy, mitochondria appeared to be both tethered to ends and bound laterally along the sides of microtubules. Mitochondrial tubules extended and retracted when attached to the tips of elongating or shortening microtubules, respectively, but translocation along established microtubules was never observed. Mitochondria that were not associated with microtubules were largely immob

SUBMITTER: Yaffe MP 

PROVIDER: S-EPMC208773 | biostudies-literature | 2003 Sep

REPOSITORIES: biostudies-literature