Metabolic transcription analysis of engineered Escherichia coli strains that overproduce L-phenylalanine.

ABSTRACT:

Background

The rational design of L-phenylalanine (L-Phe) overproducing microorganisms has been successfully achieved by combining different genetic strategies such as inactivation of the phosphoenolpyruvate: phosphotransferase transport system (PTS) and overexpression of key genes (DAHP synthase, transketolase and chorismate mutase-prephenate dehydratase), reaching yields of 0.33 (g-Phe/g-Glc), which correspond to 60% of theoretical maximum. Although genetic modifications introduced into the cell for the generation of overproducing organisms are specifically targeted to a particular pathway, these can trigger unexpected transcriptional responses of several genes. In the current work, metabolic transcription analysis (MTA) of both L-Phe overproducing and non-engineered strains usin

SUBMITTER: Baez-Viveros JL 

PROVIDER: S-EPMC2089068 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature