Hippocampal dendritic arbor growth in vitro: regulation by Reelin-Disabled-1 signaling.

ABSTRACT: The cytoplasmic adaptor protein Disabled-1 (Dab1), which is a key component of the Reelin-signaling pathway, has been suggested to be required for neuronal dendritic development. However, only data from studies on immature cultures [< or = 6 days in vitro (DIV)] and cytoarchitectural analyses of mutant mice have been used to formulate this hypothesis. Therefore, to determine if Reelin-Dab1 signaling is specifically required for neurons to develop mature dendrites in respect to length and complexity, we analyzed dendritic development in mature cultures derived from Dab1 knockout (ko) embryos. No significant differences in dendritic length or complexity between Dab1 ko and wt cultures were found at 20 DIV. An examination of dendritic development in maturing cultures found significant differe

SUBMITTER: MacLaurin SA 

PROVIDER: S-EPMC2094008 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature