A specific role for the C-terminal region of the Poly(A)-binding protein in mRNA decay.

ABSTRACT: mRNA poly(A) tails affect translation, mRNA export and mRNA stability, with translation initiation involving a direct interaction between eIF4G and the poly(A)-binding protein Pab1. The latter factor contains four RNA recognition motifs followed by a C-terminal region composed of a linker and a PABC domain. We show here that yeast mutants lacking the C-terminal domains of Pab1 display specific synthetic interactions with mutants in the 5'-3' mRNA decay pathway. Moreover, these mutations impair mRNA decay in vivo without significantly affecting mRNA export or translation. Inhibition of mRNA decay occurs through slowed deadenylation. In vitro analyses demonstrate that removal of the Pab1 linker domain directly interferes with the ability of the Pop2-Ccr4 complex to deadenylate the Pab1-bound

SUBMITTER: Simon E 

PROVIDER: S-EPMC2094065 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature