A study of 7-deaza-2'-deoxyguanosine 2'-deoxycytidine base pairing in DNA.

ABSTRACT: The incorporation of 7-deazaguanine modifications into DNA is frequently used to probe protein recognition of H-bonding information in the major groove of DNA. While it is generally assumed that 7-deazaguanine forms a normal Watson-Crick base pair with cytosine, detailed thermodynamic and structural analyses of this modification have not been reported. The replacement of the 7-N atom on guanine with a C-H, alters the electronic properties of the heterocycle and eliminates a major groove cation-binding site that could affect the organization of salts and water in the major groove. We report herein the characterization of synthetic DNA oligomers containing 7-deazaguanine using a variety of complementary approaches: UV thermal melting, differential scanning calorimetry (DSC), circular dichroi

SUBMITTER: Ganguly M 

PROVIDER: S-EPMC2094092 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature