Identification of key structural determinants of the IntI1 integron integrase that influence attC x attI1 recombination efficiency.

ABSTRACT: The integron platform codes for an integrase (IntI) from the tyrosine family of recombinases that mediates recombination between a proximal double-strand recombination site, attI and a single-strand target recombination site, attC. The attI site is only recognized by its cognate integrase, while the various tested attCs sites are recombined by several different IntI integrases. We have developed a genetic system to enrich and select mutants of IntI1 that provide a higher yield of recombination in order to identify key protein structural elements important for attC x attI1 recombination. We isolated mutants with higher activity on wild type and mutant attC sites. Interestingly, three out of four characterized IntI1 mutants selected on different substrates are mutants of the conserved aspart

SUBMITTER: Demarre G 

PROVIDER: S-EPMC2095811 | biostudies-literature | 2007

REPOSITORIES: biostudies-literature