Acclimation of Saccharomyces cerevisiae to low temperature: a chemostat-based transcriptome analysis.

ABSTRACT: Effects of suboptimal temperatures on transcriptional regulation in yeast have been extensively studied in batch cultures. To eliminate indirect effects of specific growth rates that are inherent to batch-cultivation studies, genome-wide transcriptional responses to low temperatures were analyzed in steady-state chemostats, grown at a fixed specific growth rate (0.03 h(-1)). Although in vivo metabolic fluxes were essentially the same in cultures grown at 12 and at 30 degrees C, concentrations of the growth-limiting nutrients (glucose or ammonia) were higher at 12 degrees C. This difference was reflected by transcript levels of genes that encode transporters for the growth-limiting nutrients. Several transcriptional responses to low temperature occurred under both nutrient-limitation regime

SUBMITTER: Tai SL 

PROVIDER: S-EPMC2096585 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature