Noise propagation and scaling in regulation of gonadotrope biosynthesis.

ABSTRACT: Reproductive physiology depends on the control of biosynthesis in the pituitary gonadotrope by hypothalamic gonadotropin-releasing hormone (GnRH). The responses to GnRH include activation of extracellular signal-regulated kinase (ERK) and induction of Egr1. Using population and single cell signaling assays, we investigated the signal and noise transmission through this signaling and gene circuit, analyzing data obtained from 43,775 individual cells in 40 experiments. After exposure to GnRH, phosphorylated ERK (pERK) is elevated in 50% of the cells at 1.7 (SD = 0.3) min. Studies of the cell-to-cell response showed that for both pERK and for Egr1 protein production the mean response (mu) and standard deviation (sigma) within individual cells were linearly related (sigma = kmu) and had simila

SUBMITTER: Ruf F 

PROVIDER: S-EPMC2098712 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature