Meta-analysis of Drosophila circadian microarray studies identifies a novel set of rhythmically expressed genes.

ABSTRACT: Five independent groups have reported microarray studies that identify dozens of rhythmically expressed genes in the fruit fly Drosophila melanogaster. Limited overlap among the lists of discovered genes makes it difficult to determine which, if any, exhibit truly rhythmic patterns of expression. We reanalyzed data from all five reports and found two sources for the observed discrepancies, the use of different expression pattern detection algorithms and underlying variation among the datasets. To improve upon the methods originally employed, we developed a new analysis that involves compilation of all existing data, application of identical transformation and standardization procedures followed by ANOVA-based statistical prescreening, and three separate classes of post hoc analysis: cross-

SUBMITTER: Keegan KP 

PROVIDER: S-EPMC2098839 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature