Universal primers for HBV genome DNA amplification across subtypes: a case study for designing more effective viral primers.

ABSTRACT:

Background

The highly heterogenic characteristic of viruses is the major obstacle to efficient DNA amplification. Taking advantage of the large number of virus DNA sequences in public databases to select conserved sites for primer design is an optimal way to tackle the difficulties in virus genome amplification.

Results

Here we use hepatitis B virus as an example to introduce a simple and efficient way for virus primer design. Based on the alignment of HBV sequences in public databases and a program BxB in Perl script, our method selected several optimal sites for HBV primer design. Polymerase chain reaction showed that compared with the success rate of the most popular primers for whole genome amplification of HBV, one set of primers for full length genome amplification and

SUBMITTER: Zhang Q 

PROVIDER: S-EPMC2099425 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature