Alveolar macrophages lack CCR2 expression and do not migrate to CCL2.

ABSTRACT:

Background

The recruitment of mononuclear cells has important implications for tissue inflammation. Previous studies demonstrated enhanced CCR1 and CCR5 expression and decreased CCR2 expression during in vitro monocyte to macrophage differentiation. To date, no study examined the in vivo differences in chemokine receptor expression between human peripheral blood monocytes and alveolar macrophages.

Methods

We examined the expression of these receptors in human peripheral blood monocytes and alveolar macrophages using microarray analysis, reverse-transcriptase PCR, flow cytometry and migration analyses.

Results

In contrast to peripheral blood monocytes, alveolar macrophages did not express the CCL2 receptor, CCR2, and did not migrate toward CCL2. In contrast, monocytes

SUBMITTER: Opalek JM 

PROVIDER: S-EPMC2099427 | biostudies-literature | 2007 Sep

REPOSITORIES: biostudies-literature