Nanopore-based kinetics analysis of individual antibody-channel and antibody-antigen interactions.

ABSTRACT:

Background

The UNO/RIC Nanopore Detector provides a new way to study the binding and conformational changes of individual antibodies. Many critical questions regarding antibody function are still unresolved, questions that can be approached in a new way with the nanopore detector.

Results

We present evidence that different forms of channel blockade can be associated with the same antibody, we associate these different blockades with different orientations of "capture" of an antibody in the detector's nanometer-scale channel. We directly detect the presence of antibodies via reductions in channel current. Changes to blockade patterns upon addition of antigen suggest indirect detection of antibody/antigen binding. Similarly, DNA-hairpin anchored antibodies have been studied, w

SUBMITTER: Winters-Hilt S 

PROVIDER: S-EPMC2099489 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature