Statistical tools for transgene copy number estimation based on real-time PCR.

ABSTRACT:

Background

As compared with traditional transgene copy number detection technologies such as Southern blot analysis, real-time PCR provides a fast, inexpensive and high-throughput alternative. However, the real-time PCR based transgene copy number estimation tends to be ambiguous and subjective stemming from the lack of proper statistical analysis and data quality control to render a reliable estimation of copy number with a prediction value. Despite the recent progresses in statistical analysis of real-time PCR, few publications have integrated these advancements in real-time PCR based transgene copy number determination.

Results

Three experimental designs and four data quality control integrated statistical models are presented. For the first method, external calibration c

SUBMITTER: Yuan JS 

PROVIDER: S-EPMC2099498 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature