N348I in the connection domain of HIV-1 reverse transcriptase confers zidovudine and nevirapine resistance.

ABSTRACT:

Background

The catalytically active 66-kDa subunit of the human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) consists of DNA polymerase, connection, and ribonuclease H (RNase H) domains. Almost all known RT inhibitor resistance mutations identified to date map to the polymerase domain of the enzyme. However, the connection and RNase H domains are not routinely analysed in clinical samples and none of the genotyping assays available for patient management sequence the entire RT coding region. The British Columbia Centre for Excellence in HIV/AIDS (the Centre) genotypes clinical isolates up to codon 400 in RT, and our retrospective statistical analyses of the Centre's database have identified an N348I mutation in the RT connection domain in treatment-experienced i

SUBMITTER: Yap SH 

PROVIDER: S-EPMC2100143 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature