Modulation of bacterial Type III secretion system by a spermidine transporter dependent signaling pathway.

ABSTRACT:

Background

Many gram-negative bacterial pathogens employ Type III secretion systems (T3SS) to inject effector proteins into host cells in infectious processes.

Methodology/principal findings

By screening a transposon mutant library of P. aeruginosa, we found that mutation of spuDEFGH, which encode a major spermidine uptake system, abolished the expression of the exsCEBA operon that codes for key T3SS regulators under inducing conditions (low calcium). Whole genome microarray analysis revealed that inactivation of the spermidine uptake system significantly decreased the transcriptional expression of most, if not all, T3SS genes. Consistently, the spermidine uptake mutants showed decreased expression of the T3SS genes in responding to host cell extract and attenuated cytotoxic

SUBMITTER: Zhou L 

PROVIDER: S-EPMC2110884 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature