A new in vitro strand transfer assay for monitoring bacterial class 1 integron recombinase IntI1 activity.

ABSTRACT: IntI1 integrase is a tyrosine recombinase involved in the mobility of antibiotic resistance gene cassettes within bacterial class 1 integrons. Recent data have shown that its recombination specifically involves the bottom strand of the attC site, but the exact mechanism of the reaction is still unclear. An efficient in vitro assay is still required to better characterize the biochemical properties of the enzyme. In this report we describe for the first time an in vitro system partially reproducing the activity of a recombinant pure IntI1. This new assay, which constitutes the only available in vitro model of recombination by IntI1, was used to determine whether this enzyme might be the sole bacterial protein required for the recombination process. Results show that IntI1 possesses all the

SUBMITTER: Dubois V 

PROVIDER: S-EPMC2117344 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature