CD8 single-cell gene coexpression reveals three different effector types present at distinct phases of the immune response.

ABSTRACT: To study in vivo CD8 T cell differentiation, we quantified the coexpression of multiple genes in single cells throughout immune responses. After in vitro activation, CD8 T cells rapidly express effector molecules and cease their expression when the antigen is removed. Gene behavior after in vivo activation, in contrast, was quite heterogeneous. Different mRNAs were induced at very different time points of the response, were transcribed during different time periods, and could decline or persist independently of the antigen load. Consequently, distinct gene coexpression patterns/different cell types were generated at the various phases of the immune responses. During primary stimulation, inflammatory molecules were induced and down-regulated shortly after activation, generating early cells

SUBMITTER: Peixoto A 

PROVIDER: S-EPMC2118592 | biostudies-literature | 2007 May

REPOSITORIES: biostudies-literature