Exploration of intraclonal adaptation mechanisms of Pseudomonas brassicacearum facing cadmium toxicity.

ABSTRACT: Pseudomonas brassicacearum forms phenotypic variants in vitro as well as in planta during root colonization under natural conditions, leading to subpopulations (phase I and II cells) that differ in colony morphology and production of exoenzymes/secondary metabolites. The maximal concentration of cadmium allowing both variants growth was 25 muM; however, phase II cells accumulated fivefold higher Cd than phase I cells, even though both variants showed the same growth rate and kinetics, comprising a long stasis period (50 h). The whole transcriptome analysis of both variants in response to Cd was investigated using the home-made DNA microarrays. This analysis revealed completely different adaptation mechanisms developed by each variant to withstand and grow in the presence of the toxic. A re

SUBMITTER: Pages D 

PROVIDER: S-EPMC2121137 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature