Transmembrane activator and calcium modulator and cyclophilin ligand interactor enhances CD40-driven plasma cell differentiation.

ABSTRACT:

Background

Transmembrane activator and calcium modulator and cyclophilin ligand interactor (TACI) is a receptor used by B cell-activating factor of the TNF family and a proliferation-inducing ligand (APRIL) to induce isotype switching independently of CD40 and is mutated in patients with common variable immunodeficiency.

Objective

We sought to determine whether TACI and CD40 cooperate in inducing class switch recombination and immunoglobulin production.

Methods

Naive mouse B cells were stimulated with suboptimal concentrations of anti-CD40 plus IL-4 in the presence or absence of APRIL or anti-TACI. IgG(1) and IgE production was measured by means of ELISA. mRNA for Cgamma(1) and Cepsilon germ-line transcripts, activation-induced cytidine deaminase, and mature gamma(1)

SUBMITTER: Castigli E 

PROVIDER: S-EPMC2121612 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature