Metabolic flux elucidation for large-scale models using 13C labeled isotopes.

ABSTRACT: A key consideration in metabolic engineering is the determination of fluxes of the metabolites within the cell. This determination provides an unambiguous description of metabolism before and/or after engineering interventions. Here, we present a computational framework that combines a constraint-based modeling framework with isotopic label tracing on a large scale. When cells are fed a growth substrate with certain carbon positions labeled with (13)C, the distribution of this label in the intracellular metabolites can be calculated based on the known biochemistry of the participating pathways. Most labeling studies focus on skeletal representations of central metabolism and ignore many flux routes that could contribute to the observed isotopic labeling patterns. In contrast, our approach

SUBMITTER: Suthers PF 

PROVIDER: S-EPMC2121621 | biostudies-literature | 2007 Sep-Nov

REPOSITORIES: biostudies-literature