Mitotic spindle positioning in Saccharomyces cerevisiae is accomplished by antagonistically acting microtubule motor proteins.

ABSTRACT: Proper positioning of the mitotic spindle is often essential for cell division and differentiation processes. The asymmetric cell division characteristic of budding yeast, Saccharomyces cerevisiae, requires that the spindle be positioned at the mother-bud neck and oriented along the mother-bud axis. The single dynein motor encoded by the S. cerevisiae genome performs an important but nonessential spindle-positioning role. We demonstrate that kinesin-related Kip3p makes a major contribution to spindle positioning in the absence of dynein. The elimination of Kip3p function in dyn1Delta cells severely compromised spindle movement to the mother-bud neck. In dyn1Delta cells that had completed positioning, elimination of Kip3p function caused spindles to mislocalize to distal positions in mother

SUBMITTER: Cottingham FR 

PROVIDER: S-EPMC2136752 | biostudies-literature | 1997 Sep

REPOSITORIES: biostudies-literature