Supervillin (p205): A novel membrane-associated, F-actin-binding protein in the villin/gelsolin superfamily.

ABSTRACT: Actin-binding membrane proteins are involved in both adhesive interactions and motile processes. We report here the purification and initial characterization of p205, a 205-kD protein from bovine neutrophil plasma membranes that binds to the sides of actin filaments in blot overlays. p205 is a tightly bound peripheral membrane protein that cosediments with endogenous actin in sucrose gradients and immunoprecipitates. Amino acid sequences were obtained from SDS-PAGE-purified p205 and used to generate antipeptide antibodies, immunolocalization data, and cDNA sequence information. The intracellular localization of p205 in MDBK cells is a function of cell density and adherence state. In subconfluent cells, p205 is found in punctate spots along the plasma membrane and in the cytoplasm and nucle

SUBMITTER: Pestonjamasp KN 

PROVIDER: S-EPMC2140202 | biostudies-literature | 1997 Dec

REPOSITORIES: biostudies-literature