Stu2p: A microtubule-binding protein that is an essential component of the yeast spindle pole body.

ABSTRACT: Previously we isolated tub2-423, a cold-sensitive allele of the Saccharomyces cerevisiae gene encoding beta-tubulin that confers a defect in mitotic spindle function. In an attempt to identify additional proteins that are important for spindle function, we screened for suppressors of the cold sensitivity of tub2-423 and obtained two alleles of a novel gene, STU2. STU2 is an essential gene and encodes a protein whose sequence is similar to proteins identified in a variety of organisms. Stu2p localizes primarily to the spindle pole body (SPB) and to a lesser extent along spindle microtubules. Localization to the SPB is not dependent on the presence of microtubules, indicating that Stu2p is an integral component of the SPB. Stu2p also binds microtubules in vitro. We have localized the microtu

SUBMITTER: Wang PJ 

PROVIDER: S-EPMC2140218 | biostudies-literature | 1997 Dec

REPOSITORIES: biostudies-literature