Separate functions for nuclear and cytoplasmic cryptochrome 1 during photomorphogenesis of Arabidopsis seedlings.

ABSTRACT: Cryptochrome blue-light receptors mediate many aspects of plant photomorphogenesis, such as suppression of hypocotyl elongation and promotion of cotyledon expansion and root growth. The cryptochrome 1 (cry1) protein of Arabidopsis is present in the nucleus and cytoplasm of cells, but how the functions of one pool differ from the other is not known. Nuclear localization and nuclear export signals were genetically engineered into GFP-tagged cry1 molecules to manipulate cry1 subcellular localization in a cry1-null mutant background. The effectiveness of the engineering was confirmed by confocal microscopy. The ability of nuclear or cytoplasmic cry1 to rescue a variety of cry1 phenotypes was determined. Hypocotyl growth suppression by blue light was assessed by standard end-point analyses and

SUBMITTER: Wu G 

PROVIDER: S-EPMC2141859 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature