Kinetics of DNA-mediated docking reactions between vesicles tethered to supported lipid bilayers.

ABSTRACT: Membrane-membrane recognition and binding are crucial in many biological processes. We report an approach to studying the dynamics of such reactions by using DNA-tethered vesicles as a general scaffold for displaying membrane components. This system was used to characterize the docking reaction between two populations of tethered vesicles that display complementary DNA. Deposition of vesicles onto a supported lipid bilayer was performed by using a microfluidic device to prevent mixing of the vesicles in bulk during sample preparation. Once tethered onto the surface, vesicles mixed via two-dimensional diffusion. DNA-mediated docking of two reacting vesicles results in their colocalization after collision and their subsequent tandem motion. Individual docking events and population kinetics w

SUBMITTER: Chan YH 

PROVIDER: S-EPMC2141882 | biostudies-literature | 2007 Nov

REPOSITORIES: biostudies-literature