A high throughput screen to identify secreted and transmembrane proteins involved in Drosophila embryogenesis.

ABSTRACT: Secreted and transmembrane proteins play an essential role in intercellular communication during the development of multicellular organisms. Because only a small number of these genes have been characterized, we developed a screen for genes encoding extracellular proteins that are differentially expressed during Drosophila embryogenesis. Our approach utilizes a new method for screening large numbers of cDNAs by whole-embryo in situ hybridization. The cDNA library for the screen was prepared from rough endoplasmic reticulum-bound mRNA and is therefore enriched in clones encoding membrane and secreted proteins. To increase the prevalence of rare cDNAs in the library, the library was normalized using a method based on cDNA hybridization to genomic DNA-coated beads. In total, 2,518 individual

SUBMITTER: Kopczynski CC 

PROVIDER: S-EPMC21446 | biostudies-literature | 1998 Aug

REPOSITORIES: biostudies-literature