Representational difference analysis of a committed myeloid progenitor cell line reveals evidence for bilineage potential.

ABSTRACT: In this study we have sought to characterize a committed myeloid progenitor cell line in an attempt to isolate general factors that may promote differentiation. We used cDNA representational difference analysis (RDA), which allows analysis of differential gene expression, to compare EML and EPRO cells. We have isolated nine differentially expressed cDNA fragments as confirmed by slot blot, Northern, and PCR analysis. Three of nine sequences appear to be novel whereas the identity of the remaining fragments suggested that the EPRO cell line is multipotent. Among the isolated sequences were eosinophilic, monocytic, and neutrophilic specific genes. Therefore, we tested the ability of EPRO cells to differentiate along multiple myeloid lineages and found that EPRO cells exhibited morphologic ma

SUBMITTER: Lawson ND 

PROVIDER: S-EPMC21473 | biostudies-literature | 1998 Aug

REPOSITORIES: biostudies-literature