Proteomics reveals multiple routes to the osteogenic phenotype in mesenchymal stem cells.

ABSTRACT:

Background

Recently, we demonstrated that human mesenchymal stem cells (hMSC) stimulated with dexamethazone undergo gene focusing during osteogenic differentiation (Stem Cells Dev 14(6): 1608-20, 2005). Here, we examine the protein expression profiles of three additional populations of hMSC stimulated to undergo osteogenic differentiation via either contact with pro-osteogenic extracellular matrix (ECM) proteins (collagen I, vitronectin, or laminin-5) or osteogenic media supplements (OS media). Specifically, we annotate these four protein expression profiles, as well as profiles from naïve hMSC and differentiated human osteoblasts (hOST), with known gene ontologies and analyze them as a tensor with modes for the expressed proteins, gene ontologies, and stimulants.

Results

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SUBMITTER: Bennett KP 

PROVIDER: S-EPMC2148065 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature