A novel polydnavirus protein inhibits the insect prophenoloxidase activation pathway.

ABSTRACT: Pathogens often suppress the melanization response of host insects, but the underlying molecular mechanisms are largely unknown. Here we report that Microplitis demolitor bracovirus (MdBV) carried by the wasp M. demolitor produces a protein, Egf1.0, which inhibits the phenoloxidase (PO) cascade. Egf1.0 belongs to a larger gene family that shares a cysteine-rich motif with similarities to the trypsin inhibitor-like (TIL) domains of small serine proteinase inhibitors (smapins). Gain-of-function and RNAi experiments indicated that the Egf genes are the only MdBV-encoded factors responsible for disabling the insect melanization response. Known smapins bind target proteinases in a substrate-like fashion and are cleaved at a single reactive site bond. The P1-P1' position for Egf1.0 has the seque

SUBMITTER: Beck MH 

PROVIDER: S-EPMC2148279 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature