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Crystallization and preliminary X-ray crystallographic analysis of a galactose-specific lectin from Dolichos lablab.

ABSTRACT: The galactose-specific lectin from the seeds of Dolichos lablab has been crystallized using the hanging-drop vapour-diffusion technique. The crystals belong to space group P1, with unit-cell parameters a = 73.99, b = 84.13, c = 93.15 A, alpha = 89.92, beta = 76.01, gamma = 76.99 degrees. X-ray diffraction data to a resolution of 3.0 A have been collected under cryoconditions (100 K) using a MAR imaging-plate detector system mounted on a rotating-anode X-ray generator. Molecular-replacement calculations carried out using the available structures of legume lectins as search models revealed that the galactose-specific lectin from D. lablab forms a tetramer similar to soybean agglutinin; two such tetramers are present in the asymmetric unit.

SUBMITTER: Latha VL 

PROVIDER: S-EPMC2150945 | biostudies-literature | 2006 Feb

REPOSITORIES: biostudies-literature

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Crystallization and preliminary X-ray crystallographic analysis of a galactose-specific lectin from Dolichos lablab.

Latha V Lavanya VL   Kulkarni Kiran A KA   Rao R Nagender RN   Kumar N Siva NS   Suguna K K  

Acta crystallographica. Section F, Structural biology and crystallization communications 20060127 Pt 2

The galactose-specific lectin from the seeds of Dolichos lablab has been crystallized using the hanging-drop vapour-diffusion technique. The crystals belong to space group P1, with unit-cell parameters a = 73.99, b = 84.13, c = 93.15 A, alpha = 89.92, beta = 76.01, gamma = 76.99 degrees. X-ray diffraction data to a resolution of 3.0 A have been collected under cryoconditions (100 K) using a MAR imaging-plate detector system mounted on a rotating-anode X-ray generator. Molecular-replacement calcu  ...[more]

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