Eukaryotic ribosomal RNA determinants of aminoglycoside resistance and their role in translational fidelity.

ABSTRACT: Recent studies of prokaryotic ribosomes have dramatically increased our knowledge of ribosomal RNA (rRNA) structure, functional centers, and their interactions with antibiotics. However, much less is known about how rRNA function differs between prokaryotic and eukaryotic ribosomes. The core decoding sites are identical in yeast and human 18S rRNAs, suggesting that insights obtained in studies with yeast rRNA mutants can provide information about ribosome function in both species. In this study, we examined the importance of key nucleotides of the 18S rRNA decoding site on ribosome function and aminoglycoside susceptibility in Saccharomyces cerevisiae cells expressing homogeneous populations of mutant ribosomes. We found that residues G577, A1755, and A1756 (corresponding to Escherichia co

SUBMITTER: Fan-Minogue H 

PROVIDER: S-EPMC2151042 | biostudies-literature | 2008 Jan

REPOSITORIES: biostudies-literature