Proteome analysis of human gastric cardia adenocarcinoma by laser capture microdissection.

ABSTRACT:

Background

The incidence of gastric cardiac adenocarcinoma (GCA) has been increasing in the past two decades in China, but the molecular changes relating to carcinogenesis have not been well characterised.

Methods

In this study, we used a comparative proteomic approach to analyse the malignant and nonmalignant gastric cardia epithelial cells isolated by navigated laser capture microdissection (LCM) from paired surgical specimens of human GCA.

Results

Twenty-seven spots corresponding to 23 proteins were consistently differentially regulated. Fifteen proteins were shown to be up-regulated, while eight proteins were shown to be down-regulated in malignant cells compared with nonmalignant columnar epithelial cells. The identified proteins appeared to be involved in metabo

SUBMITTER: Cheng Y 

PROVIDER: S-EPMC2151079 | biostudies-literature | 2007 Oct

REPOSITORIES: biostudies-literature