A two-phase innate host response to alphavirus infection identified by mRNP-tagging in vivo.

ABSTRACT: A concept fundamental to viral pathogenesis is that infection induces specific changes within the host cell, within specific tissues, or within the entire animal. These changes are reflected in a cascade of altered transcription patterns evident during infection. However, elucidation of this cascade in vivo has been limited by a general inability to distinguish changes occurring in the minority of infected cells from those in surrounding uninfected cells. To circumvent this inherent limitation of traditional gene expression profiling methods, an innovative mRNP-tagging technique was implemented to isolate host mRNA specifically from infected cells in vitro as well as in vivo following Venezuelan equine encephalitis virus (VEE) infection. This technique facilitated a direct characterization

SUBMITTER: Konopka JL 

PROVIDER: S-EPMC2151086 | biostudies-literature | 2007 Dec

REPOSITORIES: biostudies-literature